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Institute of Anatomy

Seahorse XFe 96

The Seahorse XF instruments analyze mitochondrial respiration and glycolysis in live cells. Seahorse XF technology uses solid-state sensors to simultaneously measure both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in every well. All platforms support injection of up to 4 unique compounds per well.

Key features of the XFe96 Analyzer:
  • 96-well plate format accommodates many conditions in a single run, for flexible assay design, dose-response studies, and screening
  • Reports real-time metabolic rates in minutes, without sample extraction or labelling
  • Four-port injection system with automated mixing function, for detection of live-cell responses to substrates, inhibitors, and other compounds in real time
  • High-sensitivity analysis of as few as 5,000 cells per well using the custom 96-well plate
  • Precision-controlled heating tray, maintains 16–42°C temperatures (12–20°C above ambient conditions), for compatibility with a variety of sample types
  • Quickly determine dependency of cellular energy production on mitochondrial substrates
  • Generate a metabolic phenotype within one hour for fast data turnaround
  • Analyze 3D samples such as spheroids and islets
  • Wave software allows easy assay protocol creation and data analysis on your desktop PC and export to common spreadsheet and graphing programs



Zheng Fan

General coordination and repair:

Edda Kastenhuber